Nystatin (NT) is an antibiotic which shows both fungistatic and fungicidal activity against a wide variety of yeasts and yeast-like fungi, including Candida albicans. Nystatins have very low permeation topically. NT also shows skin irritation by topical application. Thus the aim of present study was to increase solubility, permeability of NT and also reduce skin irritation by formulating a lyotropic liquid crystalline system. The liquid crystalline system of NT was formulated using glyceryl monooleate (GMO) / poloxamer 407 / water. The prepared liquid crystalline system was also compared with simple gel and emulgel of NT. The formulations were evaluated for phase identification, in-vitro and ex-vivo permeation study, texture characterization, skin irritation study, antifungal activity and stability studies. The optimized formulation F4 shows hexagonal phase identified by polarized light microscopy. In-vitro cumulative drug release of the optimized batch was 71.6 % and ex-vivo cumulative drug release was 50.01 % within 12 h. The antifungal inhibitory activity of optimized batch shows the highest zone of inhibition as compared to emulgel and simple carbopol gel. Skin irritation study suggested that prepared formulation shows no skin irritation upon topical administration. Furthermore the prepared optimized formulation was found to be stable.
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