Background: Mucoadhesion is an important property that helps oral drug delivery system to remain attached with\nbuccal mucosa and hence to improve the delivery of the drug. The current study was designed to achieve the thiol\nmodification of Arabinoxylan (ARX) and to develop a mucoadhesive oral film for the improved delivery of tizanidine\nhydrochloride (TZN HCl).\nMethod: Synthesis of thiolated arabinoxylan (TARX) was accomplished by esterification of ARX with thioglycolic\nacid (TGA). TARX was further used for the development of mucoadhesive oral films which were prepared by using\na solvent casting technique. Formulation of the films was designed and optimized by using central composite\ndesign (CCRD), selecting TARX (X1) and glycerol (X2) as variables. Prepared film formulations were evaluated for\nmechanical strength, ex-vivo mucoadhesion, in-vitro drug release, ex-vivo drug permeation, surface morphology and\ndrug contents.\nResults: Thiolation of ARX was confirmed by fourier transform infra-red spectroscopy (FTIR) as a peak related to\nthiol group appeared at 2516 cmâË?â??1. The claim of successful thiolation of ARX was strengthened by the presence of\n2809.003 Ã?± 1.03 Ã?¼moles of thiol contents per gram of the polymer, which was determined by Ellmanââ?¬â?¢s reagent\nmethod. From the results, it was observed that the films were of satisfactory mechanical strength and mucoadhesiveness\nwith folding endurance greater than 300 and mucoadhesive strength 11.53 Ã?± 0.17 N, respectively. Reasonable drug\nretention was observed during in-vitro dissolution (85.03% cumulative drug release) and ex-vivo permeation (78.90%\ncumulative amount of permeated drug) studies conducted for 8 h. Effects of varying concentrations of both polymer\nand plasticizer on prepared mucoadhesive oral films were evaluated by ANOVA and it was observed that glycerol can\nenhanced the dissolution as well as permeation of the drug while TARX has opposite impact on these parameters.\nConclusion: In nutshell, TARX in combination with glycerolwas found to be suitable for the development of controlled\nrelease mucoadhesive oral films of TZN HCl.
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