Background: Resveratrol effects on the prevention and treatment of colon\ncancer have been well documented recently, but low solubility, rapid\nabsorption and metabolism of resveratrol limit its beneficial effects on colon\ncancer. Designing a formulation that enhances the solubility of resveratrol,\nprotects resveratrol from oxidation and isomerization, and delivers it to the\ncolon is a priority of food and drug industry. In this study, resveratrolpolyethylene\nglycol (PEG)-loaded pectin-chitosan polyelectrolyte complex\nwas designed as a colon targeted delivery system.\nMethods: The effects of adding PEG, ultra-sonication time, pH, and pectin to\nchitosan ratio were investigated on particle size, polydispersity index (PDI),\nzeta potential by particle size analyzer, and scanning electron microscopy\n(SEM). Encapsulation efficiency (EE), release of resveratrol in simulated\ngastrointestinal fluid, and different pHs were analyzed via High Performance\nLiquid Chromatography (HPLC). Antioxidant activity was measured by (2, 2-\ndiphenyl-1-picryl-hydrazyl-hydrate) DPPH free-radical method.\nResults: Results showed that colloidal stable micro-particles (725 �± 20 nm)\nwith PDI < 0.3 and zeta potential +27 �± 2 mV was formed in the ratio of 5:1\nof pectin to chitosan w/v % after a 10-min sonication. Encapsulation\nefficiency was 81 �± 7 %. The reduction of antioxidant activity of resveratrol\nloaded micro-particles after one month was less than 13%. Micro-particles\nreleased about 33% of resveratrol in the simulated gastric and intestinal fluids.\nConclusion: Two-thirds of the loaded resveratrol in Pectin-Chitosan complex\nreached colon. The developed system had enough specification for enriching\nfruit based drinks due to remarkable colloidal stability in the pH range of 3.5\nto 4.5.
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