Cell type, morphology, and functioning are key variables in the construction of efficient ââ?¬Å?drug-vehicleââ?¬Â hybrids in magnetic drug\ndelivery. Iron-encapsulated multiwall carbon nanotubes (Fe@MWCNTs) appear as promising candidates for theranostics due to\nin situ chemical catalytic vapor deposition (c-CVD) synthesis, straightforward organic functionalization, and nanoneedle (1D)\nbehavior. Here, model hybrids were synthesized by exploring C-sp2 chemistry ((1+2)-cycloaddition of nitrenes and amidation) of\nthe outer MWCNT walls combined with anticancer agents, that is, 5-fluorouracil (5FU), purpurin (Purp), and 1,8-naphthalimide\nDNA intercalators (NIDIs), via linkers. Analyses of the Fe@MWCNT vehicles by SEM, TEM, and Raman spectroscopy revealed\ntheir morphology whileMÃ?¨ossbauer spectroscopy confirmed the presence of encapsulated ferromagnetic iron-based nanodomains.\nCytotoxicity of the hybrids was studied using a 24 h MTS assay combined with the apoptosis and life cycle assays against human\nmelanoma (Me45), colon carcinoma (HCT116+), and colon adenocarcinoma (Caco-2). The cells had different sensitivity to the\nvehicles themselves as well as to the hybrids. MWCNT-based covalent hybrids of 5FU and Purp emerged as the most promising\nsystems against Me45 and HCT116+ cell lines with the highest in vitro cytotoxicity and proapoptotic activity. Furthermore,\nnanotubes bearing 4-nitro- and 4-(N-morpholinyl)-1,8-naphthalimide DNA intercalators appear as a promising candidate for the\ntreatment of Caco-2.
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