Aim-In this study Solid Lipid Nanoparticles (SLNs) containing 5-Fluorourasil, nonsteroidal antiestrogens have been loaded by high shear hot homogenization followed by ultrasonication technique and to be used as breast cancer therapy, was prepared to decrease the dose as well as to increase the bioavailability of drug. Methods-A 32 factorial design was utilized to optimize the formulation where the concentration of lipid stearic acid (X1) and concentration of surfactant lipoid (X2) were taken as independent variables. 5-Fluorourasil loaded SLNs seem to have dimensional properties useful for oral administration. SLN was characterized by Differential Scanning Calorimetery (DSC), morphology, in vitro drug release, Zeta Potential and Particle Size. Results-Preliminary study of anticancer activity in vitro, carried out on MCF-7 cell line (human breast cancer cells), demonstrated that SLNs, containing 5-Fluorourasil showed an antitumoral activity comparable to free drug. 5-Fluorourasil loaded SLNs seem to have dimensional properties useful for oral administration. Polynomial equations and response surface plots were generated for all dependent variables using multiple regression analysis. The results of characterization studies strongly support the potential application of 5-Fluorourasil-loaded SLNs as a carrier system. Conclusion-The SLN presented here are well suited for certain drug delivery applications, particularly breast cancer therapy.
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