A rice grain’s proximate composition determines its nutritional potential. Macronutrient quantification is essential to identify superior genotypes and direct breeding efforts to reach more people who are vulnerable. Conventional methods to determine proximate composition are highly accurate; however, they remain time-consuming, costly, and destructive. Near-infrared (NIR) spectroscopy enables proximate composition analysis in a non-destructive, rapid, inexpensive, and practical manner, providing results similar to well-established conventional methods. This study aimed to evaluate the feasibility of NIRs-based selection to identify more nutritious rice genotypes. A collection of 155 rice genotypes grown in Southern Brazil was used. After harvest, grains were hulled, polished, and milled. NIRs was used to determine moisture, starch, protein, fat, ash, and fiber contents in rice flour. It was possible to differentiate genotypes with higher and lower levels of the investigated components. Similar and distinct values were observed in comparison to other studies, indicating the accuracy of NIRs and the effect of genotype and environment, respectively. Starch is correlated negatively with protein and fat, preventing the identification of genotypes with high levels of these three components. PCA enabled the separation of the genotypes but highlighted the complexity of sample distribution. NIRs is an effective and accurate method to determine the proximate composition of rice, enabling the selection of more nutritious genotypes.
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