Present study investigated the role of ZML extracts in alloxan induced hyperalgesia in mice. Alloxan (150 mg/kg; i.p) induces diabetes in mice and long standing hyperglycemia (for 21 days) causes diabetic neuropathy. 30 mice were divided in 6 groups, each group containing 5 mice: Non diabetic group, diabetic group and ZML treated groups (Ethanol and Acetone extract, 100 and 250 mg/kg respectively). Mice were orally administered with ZML extract daily for 21 days starting from 15th day after alloxan administration. Neuropathic analgesia was assessed by tail flick, hot plate and tail immersion methods Diabetic mice showed increased plasma glucose concentration, serum nitrite concentration and significant pain hypersensitivity as compared to diabetic mice. Supplementation with ZML extracts, significantly decrease the pain intensity and serum nitrite concentration. The decrease in both pain perception and serum nitrite concentration is more significant with acetone extract of ZML (100 mg/kg and 250 mg/kg). This study provides therapeutic potential of ZML extracts attenuates diabetic neuropathic pain in a dose dependent manner. The exact mechanism of pain relieving is not clear, hence further studies are required to confirm the mechanism of action and responsible chemical constituents for this activity.
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