To investigate the effect of polyherbal formulation on cigarette smoke extract induced chronic obstructive pulmonary disease in mice model. Two formulations A and B were prepared from the methanolic extracts of Ocimum sanctum, Curcuma longa, Eugenia caryophyllus and Elettereria cardamom. The cigarette smoke extract (CSE) (20 µl) was administrated by intranasal route to the mice under anesthesia for inducing COPD. After induction of COPD, the mice were divided for treatment into five groups. Group I was treated with saline only, group II was treated with CSE only, group III was treated with formulation A which was given orally, group IV was treated with formulation B through intranasal route and group V was treated with dexamethasone as standard. Throughout the study the body weights were measured. On 28th day bronchoalveolar lavage fluid (BALf) was collected and total leukocyte count (TLC) and differential leukocyte counts (DLC) were performed. The formulations A and B have shown significant anti-inflammatory activity in COPD. The macrophages and neutrophils count were decreased more for formulation B compared to formulation A. The body weights were increased with formulations A and B treated groups where compared to control group. The study demonstrated that intranasal route has indicated great impact than orally treated group among these two combinations.
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