The ethnic minorities of Rajasthan use the bark of E. ganitrus as a traditional medicine for curing epilepsy. This study was performed to explore the protective effect of E. ganitrus in epileptic mice. Swiss mice were fed with the different doses (1, 10, 100, 500, 1000 and 2000 mg/kg) of ethanolic extract of E. ganitrus and assessed for toxicity parameters for acute toxicity study. Psychomotor activities of ethanolic extract of barks of E. ganitrus for 100 and 150 mg/kg doses (EGEE100 and EGEE150) were performed in mice by using various tests like actophotometer, open field, rota-rod and grip strength tests with criteria of evaluation was locomotor activity and fall of time. After 60 min. of EGEE100 and EGEE150 treatment, epilepsy was induced in mice either by subcutaneous injection of PTZ (45 mg/kg) or Via MES and all mice were observed for tonic flexion, tonic extension, clonic jerking, stupor and recovery phases. EGEE100 and EGEE150 doses were selected as per acute toxicity study and LD50 of was found to be 850 mg/kg. Psychomotor tests for EGEE 100 and EGEE 150 in mice showed CNS depressant and muscle relaxant effects. EGEE 100 and EGEE 150 in PTZ and MES induced convulsion models of mice showed that both the extracts of E. ganitrus displayed absence of tonic extension time, it concluded that these treatments exhibited antiepileptic effect in mice. EGEE100 and EGEE150 has shown ameliorative effect in epileptic mice both in PTZ and MES induced convulsion model.
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