The aim of the study is to determine the pharmacognostical characters of leaves of Cissampelos pareira Linn along with toxicological study of its hydro-alcoholic extract. The pharmacognostical characters were determined in terms of organoleptics study, physico-chemical parameters, preliminary phytochemical investigation and thin layer chromatography (TLC). The toxicological profile was determined using acute oral toxicity study, OCED guideline 423. The Organoleptic characters of the leaves revealed that it is green in color with Ovate, reniform or orbicular shape, bitter taste and aromatic odour. The physico-chemical analysis revealed total ash; water soluble ash, acid insoluble ash and sulphated ash values to be 14.8 ± 0.29%, 7.43 ±0.24 %, 2.38 ± 0.17% and 1.5 ± 0.18 % respectively. The water and alcohol extractive values to be 7.48±0.18 % and 3.66 ± 0.21 %. The phytochemical screening revealed the presence of alkaloids, flavonoids, steroids, tannins, proteins, reducing sugar, phenols and coumarins in hydro-alcoholic extract and presence of steroids, Terpenoids, fixed oil and fats in petroleum ether extract of leaves of Cissampelos pareira. The TLC of hydro-alcoholic leaves extract of Cissampelos pareira indicated the presence of alkaloids, flavonoids, steroids, terpenoids, tannins and phenolic compound. The acute oral toxicity study of hydro-alcoholic leaves extract of Cissampelos pareira revealed that the extract is safe till 2000 mg/kg and the dose range 80 – 400 mg/kg was found to be safe and effective.
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