Screening of phytochemical constituents and in-vitro antioxidant activity of methanol extract of root of Heracleum candolleanum (Wight et Arn.) Gamble, family Apiaceae was investigated. The preliminary phytochemical analysis was done to find out the presence of different bioactive compounds. In-vitro antioxidant studies of methanol extract of roots were carried out by reducing method, DPPH, super oxide, nitric oxide and hydroxyl radical scavenging method. Extract showed the presence of phytoconstituents such as coumarins, alkaloids, sterols/terpenoids, phenols, resins, glycosides, reducing sugars, carbohydrates and volatile oils. TLC spots of the extract showed the presence of different metabolites and the total phenolic content of the extract was found to be significant. Reducing power and in-vitro assays of methanol extract established the potential antioxidant activity. The finding suggests that the methanol extract of H. candolleanum is a rich source of bioactive phytocompounds, with significant antioxidant activity in different in-vitro models and could be a potent source of natural antioxidant.
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