The study was designed to investigate the possible actions of alcoholic extract from bark of Acacia nilotica Deile on antidiabetic activity and antioxidant defense in alloxan induced diabetic rats. The alcoholic extract were further fractionated through solvent-solvent fractionation and the effect of sub fractions ethyl acetate, n butanol and remaining aqueous fraction were also investigated. The blood level was measured according to glucose oxidase method. Glibenclamide was used as reference drug at dose of five mg/kg. The antihyperglycemic effect of ethyl acetate fraction was accompanied by significant decrease in blood glucose levels and brought serum lipids to almost normal in diabetic rats. The ethyl acetate fraction possessed maximum capability to inhibit the lipid peroxidation, increase glycogen content and activate the antioxidant enzymes. Based on these results, it was observed that treatment with ethyl acetate fraction of Acacia nilotica for 21 days at dose of 100 mg/kg body weight protects from oxidative stress induced by alloxan in rats and thus helps in evaluation of the traditional claim on this plant.
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