The anticancer-cytotoxic activities of ethanolic extract of Pleiospermium alatum were tested under invitro conditions in different cancer cell lines like MCF-7, MDAMB-231,Hela, HepG2 cell lines. The active constituents carbohydrates, steroids, Alkaloids, Tannins, Flavanoids, Saponins, gums and mucilage. The ethanolic extract of Pleiospermium alatum sowed a good cytotoxic activity ( 60%,60%,85% and 65%) in Hela , HepG2, MCF-7, MDAMB-231 cells at 24 hrs respectively. In this invitro studies the normal cells are act as a positive control. The ethanolic extract of Pleiospermium alatum shows maximum percentage of 85% after 24 hrs. From this study, it can be concluded that the phytoconstituents of Pleiospermium alatum have significant anti cancer-cytotoxic activity on Hela , HepG2, MCF-7, MDAMB-231 cells.
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