Multiphoton laser microscopy is a new, non-invasive technique providing access to the skin at a cellular and subcellular level, which\r\nis based both on autofluorescence and fluorescence lifetime imaging.Whereas the former considers fluorescence intensity emitted\r\nby epidermal and dermal fluorophores and by the extra-cellular matrix, fluorescence lifetime imaging (FLIM), is generated by\r\nthe fluorescence decay rate. This innovative technique can be applied to the study of living skin, cell cultures and ex vivo samples.\r\nAlthough still limited to the clinical research field, the development ofmultiphoton laser microscopy is thought to become suitable\r\nfor a practical application in the next few years: in this paper, we performed an accurate review of the studies published so far,\r\nconsidering the possible fields of application of this imaging method and providing high quality images acquired in the Department\r\nof Dermatology of the University of Modena.
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