Analysis of Menthol obtained from extraction from the formulation was done by the proposed method. Separation of drug was achieved employing toluene: ethyl acetate (93:7) as the mobile phase on a pre-coated silica gel aluminium plate 60F254 using vanillin-sulphuric acid as colouring reagent. Quantitation was carried out in absorbance mode at 460 nm. The method showed good linearity in the range of 5-25 μg/band, method was found being precise on intra-day and inter day basis as the average % CV value for the determination of menthol was found to be 0.223 – 0.356 and 0.313 - 0.463 respectively. The method showed % mean recovery of 98.74±0.43% and 98.62±0.49 for cold rub vaporizing ointment and menthosil nasal inhaler respectively. The LOD and LOQ for estimation of menthol were found to be 0.33 and 1.00 μg/band respectively. The method was validated for linearity, accuracy, precision and specificity.
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