The aim of present work was to develop an accurate, simple and cost effective UV spectrophotometric method for estimation of lumefantrine. This method was based on area under curve of UV spectrum between 290 to 310 nm and validated as per ICH guideline Q2 (R1). The method has followed linearity in the range of 10-40 μg/ml. The value of correlation coefficient was 0.9992. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method is precise. Results of the recovery studies (99.09% to 99.40%) shows accuracy of the method. LOD and LOQ were calculated as 0.77 μg/ml and 2.34 μg/ml, respectively. The developed method can be used for routine estimation of lumefantrine in bulk and various dosage forms.
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