A simple, sensitive, accurate, rapid and economical spectrophotometric methods have been developed and validated for the estimation of metronidazole (MET) and ofloxacin (OFX) in pH 6.8 phosphate buffer (simulated intestinal fluid, SIF). The drugs were estimated from the bulk and pharmaceutical dosage forms. The max were found to be 321 and 288 nm for MET and OFX respectively alone and in combination. The proposed methods have permitted the quantification of MET and OFX over linearity in the range of 0.5-20 and 5-50 g/mL for their individual and simultaneous estimation respectively. The methods were validated as per USP and ICH guidelines for the parameters such as recovery, precision, ruggedness and repeatability. The low RSD values of less than 1.14% indicated that the proposed methods were accurate. The RSD values for intra-day and inter-day variation were obtained between 0.49-1.38% and 0.34-1.59% respectively. The estimated drugs from the formulations containing MET and OFX alone were between 396.1(±3.81) to 403.5(±2.96)mg and 198.9(±2.64) to 203.66(±1.83)mg respectively. Further the drug concentrations in the formulations containing MET and OFX in combination were between 198.69(±2.72) to 202.59(±1.55)mg respectively. The low RSD value of less than 1.37% indicate that drugs from marketed tablet formulations can be analyzed using the proposed methods. The methods could be aimed at estimating MET and OFX in SIF containing pH 6.8 phosphate buffer for regular quality control testing.
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