A reversed phase high performance liquid chromatography method (HPLC) coupled with a diode array; UV spectrometer was developed for the quantitative and qualitative standardization of salicin from Salix alba L. Salicin were separated on an phenomenox C18 column (250 mm × 4.6 mm I.D., 5µm), with a mobile phase composed of 0.1 % ortho-phosphoric acid (OPA) in water and acetonitrile, under gradient elution. The method was shown to be linear (r2 > 0.99), repeatable with instrumental precision < 2.0 and intra-assay precision < 2.0 (% CV, percent coefficient of variation), precise with intra-day variation < 2.0 and inter-day variation < 2.0 (% CV, percent coefficient of variation) and sensitive over the calibration range for salicin. Salicin is fully recovered in the presence of normal condition (recovery rates > 95%). Statistical analysis proves that the proposed method was more accurate, selective, reproducible and repeatable for the estimation of the salicin from Salix alba.
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