A new, simple, sensitive, selective, precise and robust reverse phase high pressure liquid chromatographic (HPLC) method was developed and validated for the determination of meloxicam (ME) and thiocolchicoside (THIO) in tablet and other pharmaceutical dosage forms. Analysis was carried out on Shimadzu HPLC system with kromasil C18 column (180 x 4.6 mm i.d, 5 μm particle size) using isocratic mode of methanol: acetonitrile: 0.1% v/v hexane sulfonic acid in HPLC grade water (15:10:65) as mobile phase with flow rate of 0.9 ml min-1. The detection was carried out using UV detector set at 254 nm by an injection volume of 20 μl and entire separation was carried out at 35°C column temperature. For this method, Beer’s law is obeyed in the concentration range of 4.0 to 20.0 μg ml–1 of meloxicam and 2.0 to 10.0 μg ml–1 of thiocolchicoside having linear regression equation of R-values of 0.994 and 0.996 respectively. The mean percent recoveries were found to be in the ranges of 100.43% - 101.67% for meloxicam and 99.00% - 101.62% for thiocolchicoside. The developed method has been successfully applied for the analysis of both the drugs in bulk and pharmaceutical formulations. The method was validated as per ICH guidelines by determining its specificity, accuracy, precision, linearity and range, ruggedness, robustness and system suitability.
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