The simple, accurate, precise, reproducible and economic UV spectroscopic methods of quinine and ciprofloxacin have been developed for the simultaneous estimation. The method A-dual wavelength, method B-area under curve and method C-absorption ratio method were developed by using JASCO double beam UV Spectrophotometer (model-V630). As simultaneous estimation was carried out, measurement of absorbance at two wavelengths 233 nm and 272 nm which shows λmax values of quinine and ciprofloxacin respectively in distilled water. Quinine and ciprofloxacin shows linearity at selected wavelengths and obeys beers law in the concentration range of 5-30 µg/ml with good correlation coefficient 0.998 and 0.991 respectively. For the area under curve the wavelength selected for quinine was from 227 nm to 237 nm and for ciprofloxacin 264 nm to 279 nm which gives peak area for quinine 0.370041 and for ciprofloxacin 3.60596. For the method C involved formation of isoabsorptive point at 246 nm.
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