A sensitive, accurate, precise and reproducible high performance liquid chromatographic (HPLC) method was developed and validated for the analysis of triamterene and hydrochlorothiazide combination in tablet dosage form. Chromatographic separation was achieved under isocratic condition on a Thermo Hypersil BDS, C18 column (250 mm x 4.6 mm, 5 μm) utilizing a mobile phase of acetonitrile:sodium dihydrogen phosphate buffer of pH 5.5 in the ratio of 20:80 (v/v) at a flow rate of 1 ml/min with UV detection at 325 nm. The retention time of hydrochlorothiazide and triamterene was 5.1 and 9.8 min respectively. The developed method has followed linearity in the range of 7.5-37.5 μg/ml for triamterene and 5-25μg/ml for hydrochlorothiazide. The value of correlation coefficient was 0.998 and 0.998 respectively. Satisfactory values of percent relative standard deviation for the intra-day and inter-day precision indicated that method was precise. Results of the recovery studies (95% to 105%) showed accuracy of the method. LOD and LOQ were calculated as 0.137 μg/ml and 0.416 μg/ml for triamterene and 0.105 μg/ml and 0.318 μg/ml for hydrochlorothiazide respectively. The developed method can be used for routine estimation of triamterene and hydrochlorothiazide in tablet dosage forms.
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