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Inventi Impact: Pharm Analysis & Quality Assurance

Articles

  • Inventi:ppaqa/15936/15
    Development and Validation of TLC Densitometric Method for\nthe Simultaneous Estimation of Metoprolol Succinate and\nHydrochlorothiazide in Tablet Dosage Form
    15-Jul-2015 Research 2015 : July - September
    Mahesh H Kolhe*, Ramesh D Bhusal, Pranit P Hajare, Swapnil R Chavan, Amol S Dighe

    Accurate, specific, precise and robust TLC densitometric method has been developed for simultaneous estimation of\nmetoprolol succinate (METO) and hydrochlorothiazide (HCTZ) in tablet dosage form. The chromatographic separation was\nperformed on precoated silica gel TLC 60 F254 plates using toluene: methanol: ethyl acetate: glacial acetic acid (7:1.5:1:0.5\nv/v/v/v) as mobile phase. This system was found to give compact bands for metoprolol succinate and hydrochlorothiazide (RF\nvalues 0.15 and 0.35 respectively). Densitometric analysis of metoprolol succinate and hydrochlorothiazide were performed at\n230 nm. Regression analysis data for the calibration plots were indicative of good linear relationships between response and\nconcentration over the range 1000-5000 ng/band for metoprolol succinate and 500-2500 ng/band for hydrochlorothiazide. The\nmethod was validated as per ICH guidelines for accuracy, precision, LOD, LOQ and robustness.

    How to Cite this Article
    Mahesh H Kolhe, Ramesh D Bhusal,\nPranit P Hajare et al. Development and Validation of TLC\nDensitometric Method for the Simultaneous Estimation\nof Metoprolol Succinate and Hydrochlorothiazide in\nTablet Dosage Form. Inventi Impact: Pharm Analysis &\nQuality Assurance, 2015(3):131-134, 2015.
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