To develop a simple, sensitive and accurate HPTLC densitometric method for the quantitative standardization of Glycyrrhizin from in-house herbal nutritive supplement. Quantification of glycyrrhizin was done on an aluminum plates precoated with silica gel F254 plate with mobile phase composed of n-butanol:water: glacial acetic acid (10:4:2 v/v/v). Developed plates were scanned and quantification was done at wavelength of maximum absorption 251 nm. Rf value for glycyrrhizin was found to be 0.40±0.01, under the above mentioned experimental conditions. Method was shown to be linear (r2 > 0.996), repeatable with system precision (< 3.0), method precision (< 2.0) and intermediate precision (< 2.0) and sensitive over the calibration range for glycyrrhizin. Glycyrrhizin was fully recovered in the presence of normal condition in the range of 91.60%-104.99%. Statistical analysis proves that the proposed method is more selective, reproducible and repeatable for the estimation of the Glycyrrhizin from in-house herbal nutritive supplement.
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