In the present study a simple precise, rapid and reproducible reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated and the degradation behaviour of albendazole under different stress conditions were performed. The chromatographic separation was carried out by using reverse phase stainless steel column of 4.6×250 mm hypersil C18 HPLC column with 5 µm (particles packing) held in thermostat at 40°C. The mobile phase consisted of acetonitrile/water (80/20 v/v), with a flow rate of 0.8 ml/ min and UV detection at 294 nm and injection volume 20 ml was maintained. The retention time was found to be 5.472 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to international conference on harmonization ICH Q2 (R1) guidelines. The linearity was observed in the range of 10-100 µg/ml with correlation coefficient, r = 0.9935. Albendazole active substance was subjected to forced degradation to demonstrate the stability indicating power of the HPLC method. Acidic, basic oxidative degradation were used to assess the stability of the method. The drug was found to degrade in all studied conditions but the extent of degradation was different. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of albendazole.
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