A highly selective, simple, sensitive and accurate high performance liquid chromatography method has been developed and validated for the simultaneous estimation of prazosin and H2 receptor antagonists (cimetidine, famotidine & ranitidine) in the bulk material, pharmaceutical formulation and human serum. The declaration of peaks were achieved on a Nuclosil®, 100-10, C-18 column (250×4.6mm, 10 micron) using methanol:water:acetonitrile (60:45:5 v/v, pH 3.83) as the mobile phase at a flow rate of 1mL min-1 effluents were monitored at 240 nm with a UV detector. In isocratic mode, the elution times of H2 receptor antagonists and prazosin were 2.88 ± 0.01 and 4.12 ± 0.02 minutes respectively. The, assay can be used as a routine analysis as the total eluting time for the two components is less than ten minutes. Validation of the proposed method was carried out as per International Conference on Harmonization (ICH) guidelines. The assay was linear from 2.5 to 50 μg mL-1 for prazosin and from 20 to 200 μg mL-1 for H2 receptor antagonists. The method was selective, precise, and accurate and can be used for routine analysis of pharmaceutical preparations, quality control and clinical laboratories.
Loading....