A simple, rapid and precise reverse phase ultra-performance liquid chromatographic (RP-UPLC) method was developed and subsequently validated for simultaneous estimation of thiocolchicoside and lornoxicam in bulk drug and in a tablet dosage form. The method is based on ultra performance liquid chromatography (UPLC) on a reversed phase column, Waters Acquity BEH C18 (100 × 2.1 mm, 1.7 μm). The separation was carried out using a mobile phase containing a buffer and methanol (35:65 v/v), was pumped at a flow rate of 0.2 ml/min, column temperature at 35℃ using UV-detection at 298 nm. Both the drugs were well resolved on the stationary phase and the retention times were around 0.982 minute for Thiocolchicoside and 1.291 minute for lornoxicam. The method was validated and shown to be linear for both the drugs. The correlation coefficients for thiocolchicoside and lornoxicam are 0.998 and 0.997 respectively.
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