A simple, rapid, precise and accurate reversed phase high performance liquid chromatographic method has been developed for the simultaneous determination of nateglinide in combination with metformin. This method uses an ODSA C18 analytical column, a mobile phase acetonitrile and buffer containing 50 mM ammonium acetate in the ratio 60:40 (v/v), and pH adjusted to 4.8 with orthophosphoric acid. The instrumental settings are a flow rate of 1.0 ml/min and detector wavelength at 263 nm. The retention times for nateglinide and metformin are 3.55 and 2.31 min, respectively. The method is validated and shown to be linear. The linearity range for nateglinide and metformin are 20-560 µg/ml respectively. The percentage recovery for nateglinide and metformin are 98.80-100.48 and 99.71-100.56 % respectively. The correlation coefficients of nateglinide and metformin are 0.999 and 0.999, respectively. The relative standard deviation for six replicates is always less than 2%.
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