A simple, sensitive, precise & stability indicating RP-HPLC method was developed & validated for the determination of Tramadol hydrochloride in tablet dosage form. The chromatographic conditions comprised of column XTerra® C 18 column (250 mm, id 4.6 mm, 5 µm),Water Ireland using mobile phase phosphate buffer : acetonitrile (75:25 v/v) at a flow rate of 1.5 ml/min at an ambient temperature. The retention time of tramadol was 6.49 min. Tramadol hydrochloride was subjected to acid & alkali hydrolysis, oxidation, photochemical determination & thermal degradation. The degraded product was well separated from the drug. The linear regration analysis data for the calibration plots showed regration value 0.9999 in the concentration range 200.60-601.80.The value of slope & intercept were 20.87 & -6.87 respectively. The method was validated for precision, recovery, ruggedness & robustness. The drug undergoes degradation under acidic, basic, photochemical & thermal.
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