A simple, economic, accurate reverse phase isocratic RPHPLC method was developed for the Simultanious estimation of Gliclazide 80mg & Rosiglitazone 2mg in Tablet dosage form. The quantification was carried out using Symmetry C18 (4.6 x 100mm, 5µm, Make:XTerra) with UV detected at 229 nm. The elution was achieved isocratically with a mobile phase comprises of mixture of buffer 500 mL (50%) and 500 mL of Acetonitrile HPLC (50%). The flow rate was 0.8ml/min The procedure was validated as per ICH rules for Accuracy, Precision, Detection limit, Linearity, Reproducibility and Quantitation limit. The linearity concentration range was 20-100ppm for Gliclazide and 10-50ppm for Rosiglitazone with the correlation coefficient of 0.999. The percentage recovery for Gliclazide & Rosiglitazone was found to be 100.6% and 101.2% respectively. Limit of detection values were found to be 0.002mcg/ml and 0.001mcg/ml respectively for Gliclazide & Rosiglitazone. Limit of quantitation values were found to be 0.009mcg/ml and 0.006mcg/ml respectively for Gliclazide & Rosiglitazone. The method has been successfully used to analyze commercial solid dosage containing Gliclazide & Rosiglitazone with good recoveries and proved to be robust.
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