A simple, accurate and precise dual wavelength UV spectrophotometric method was developed for simultaneous determination of fluoxetine HCl and olanzapine in combined pharmaceutical dosage forms. The absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. During selection of two wavelengths the interfering component shows same absorbance while the component of interest shows significant difference in absorbance with concentration. The literature review reveals that there is no dual wavelength method was developed for this combination of drugs, hence this method was developed. The wavelengths selected for determination of fluoxetine HCl were 240 nm and 260.24 nm, whereas, the wavelengths selected for determination of olanzapine were 215.29 nm and 228.94 nm. Acetonitrile, methanol and distilled water were taken as the solvents. The Beer's law was obeyed in the concentration range of 4–20 μg/ml for fluoxetine HCl and 1-5 μg/ml for olanzapine. Correlation coefficient was found to be 0.9966 and 0.9908 for fluoxetine HCl and olanzapine, respectively for dual wavelength method. Accuracy of method was found between 98.0-102.0%. The precision (intra-day, inter-day and analyst to analyst) of method was found within limits (%CV<2). LOD was found to be 0.23 μg/ml and 0.125 μg/ml for fluoxetine HCl and olanzapine respectively and LOQ was found to be 0.708 μg/ml and 0.41 μg/ml for fluoxetine HCl and olanzapine respectively. The proposed method was successfully applied to determination of these drugs in laboratory-prepared mixtures and commercial tablets.
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