A simple high-performance thin-layer chromatographic (HPTLC) method has been developed and validated for the quantitative determination of silybin, a flavanol, which is the major active constituent of pharmacologically important favanolignane group, silymarin in Silybum marianum L and its homeopathic mother tincture. The method involves the separation and quantification of the analyte on silica gel 60 GF254 HPTLC plates with visualisation under UV and scanning at 290 nm. Using this technique, the silybin content of the seeds of the said plant and its homeopathic mother tincture has been determined.
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