An accurate, precise and ecofriendly spectrophotometric method is presented for the determination of Cefixime based on the formation of a yellow colour product with ninhydrin in the presence of bicarbonate with an absorption maximum at 438 nm. The reaction proceeds quantitatively at 97 ± 1°C in 15 min. The calibration curve is linear over the range of 45-65 μg/ml and is described by the regression equation A = (-) 0.858 + 0.021 C with a regression coefficient (r) of 0.9987 (n = 5). The calculated molar absorptivity and Sandell sensitivity values are 4.1536 x 10 3 L/mol/cm and 0.0072 μg/cm2, respectively. The limits of detection (LOD) and quantification (LOQ) calculated as per ICH guidelines are 1.13 and 3.40 μg/ml, respectively. The within-day accuracy expressed as relative error was better than 2.5% with precision (RSD) ranging from 1.02 to 1.93%. The between-day accuracy ranged from 1.5-3.0% with a precision less than 4%. Accuracy was also checked by placebo blank and synthetic mixture analyses besides a recovery study via standard addition procedure.
Loading....