Easy, sensitive and consistent uv-spectroscopic method has been developed for simultaneous estimation and validation of the resveratrol and quercetin in topical gel formulation. The in-house RES-QUE hydrogel was formulated by using carbopol as gelling agent. Resveratrol and quercetin has absorption maxima of 306 nm and 376 nm respectively. The developed method shows linearity range of 1-6 μg/ml for resveratrol and 3-8 μg/ml for quercetin. The correlation coefficient was 0.9992 for resveratrol and 0.998 for quercetin. LOD and LOQ were found to be 0.183 and 0.555 for resveratrol and 0.323 and 0.979 for quercetin. The % recovery was found to be 99% -101% for both resveratrol and quercetin indicating proposed method is accurate and precise for simultaneous estimation of resveratrol and quercetin in gel.
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