Standardization is an important aspect to ensure quality and composition of herbal formulations which also confirm optimum levels of active principles, along with traditional approaches there are various analytical techniques also available for the standardization of herbal products like HPLC, HPTLC and UV-Visible Spectroscopy etc. As per herbal analysis concern HPTLC has recently emerged as an important analytical technique for fingerprints and quantification of marker compounds. The present study involves development of fingerprinting HPTLC methods for the quantification of an ayurvedic formulation takrarishta using piperine as standard. The HPTLC method was found to be simple, precise and accurate for the routine laboratory analysis of ayurvedic formulation takrarishta. Analysis represents separated spot of piperine over HPTLC plate and densitometry chromatogram of piperine represented in Figure 2. The results showed linearity and correlation coefficient within the range of concentration (2 μg/ml-10 μg/ml). From the results it can be observed that the piperine can be easily detected as marker compound in formulation.
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