A simple stability indicating reversed-phase HPLC method was developed and subsequently validated for simultaneous estimation of diacerein (DCN) and aceclofenac (ACF) present in pharmaceutical tablet dosage forms. The proposed RP-HPLC method utilizes a Lichrospher 100 C-18 RP column (250 mm × 4mm × 5 µm) in a isocratic separation mode with mobile phase consisting of acetonitrile and phosphate buffer in the proportion of 50:50 % (v/v), pH adjusted to 6.5 using 0.1 % v/v ortho phosphoric acid delivered at a flow rate 0.8 ml/min and the effluent was monitored at 254 nm. The retention time of DCN and ACF was found to be 2.50 and 3.35 min respectively. DCN, ACF and their formulation were exposed to acidic, alkaline, photolytic, and oxidative stress conditions, and the stressed samples were analyzed by the proposed method. The described method was linear over a range of 0.05-50µg/ml for DCN and 0.2-100 µg/ml for ACF. The mean percentage recoveries were 101.28 and 99.81 for DCN and ACF respectively. F-test and t-test at 95% confidence level were used to check the intermediate precision data obtained under different experimental setups; the calculated value was found to be less than critical value.
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