A simple reverse phase liquid chromatographic method has been developed and validated for simultaneous determination of losartan potassium and hydrochlorothiazide in bulk and tablet dosage form. The separation was carried out using a mobile phase of acetonitrile: buffer: water taken in 25:65:10 %v/v (adjust pH 2.8 with orthophosphric acid). The column used was reverse phase C18 column (Purospher® STAR Hibar®C18, 4.6 mm x 25 cm, 5 µ particles packing) held at 25°C with flow rate of 1.2 ml min using UV detection at 242 nm. The described method was linear over a concentration range of 30-70 μg/ml and 7.5-17.5 μg/ml for the assay of losartan potassium and hydrochlorothiazide respectively. The retention times of losartan potassium and hydrochlorothiazide were found to be 2.89 and 3.96 min respectively. The limit of quantification (LOQ) for losartan potassium and hydrochlorothiazide were found to be 0.025574 μg/ml and 0.03193 μg/ml respectively and the limit of detection (LOD) values were found to be 0.00844 μg/ml and 0.01054 μg/ml respectively for losaratan potassium and hydrochlorthiazide. This study showed that the proposed RP-HPLC method is simple, rapid, precise, economical and accurate, which is useful for the routine determination of losartan potassium and hydrochlorothiazide bulk drug and tablet dosage form.
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