Zero-order and Area Under Curve [AUC] UV-Spectrophotometric methods have been developed and validated for the estimation of Tenofovir disproxil fumarate in bulk and its tablet formulations. The solutions of standard and sample were prepared in water. Tenofovir disproxil fumarate was estimated at 260 nm for the zero order UV-Spectrophotometric method, while area under curve for the zero order spectrum in between 250 nm to 270 nm was measured. Beer’s law was obeyed in the concentration range of 5-30 μg/ml with r2 value 0.999 for zero order. In AUC method, beer’s law was obeyed in the concentration range of 5-30 μg/ml with r2 value 0.999. These methods were tested and validated for various parameters according to ICH guidelines. The proposed methods were successfully applied for the determination of Tenofovir disproxil fumarate in tablet formulations.
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