The method discussed in the present work provides a convenient, precise and accurate way for simultaneous analysis of cilnidipine (CIL) and metoprolol succinate (METO) in its bulk and pharmaceutical dosage form by UV spectrophotometric and RP-HPLC methods. Absorbance maxima of CIL at 240 nm and METO at 223 nm were selected for the analysis. With respect to UV-spectroscopic method, regression analysis shows linearity over the concentration range 1-12 µg/ml for CIL and 3-50 µg/ml for METO with respective correlation co-efficient of 0.999 for both the drugs. The assay for CIL and METO was found to be 90.06±1.7 and 97.57±1.13 respectively. The %RSD value for both CIL and METO was found to be less than 2%. In this study the simultaneous equation of CIL and METO was carried out by simultaneous equation satisfactorily. With respect to RP-HPLC method, HPLC conditions were optimized to obtain an adequate separation of eluted compounds. The system Methanol: Buffer (87:13) with pH 6.9 and 1.0 ml/min flow rate is quite robust. The optimum wavelength for detection was 240 nm and 223 nm at which better detector response for CIL and METO was obtained respectively. The average retention time for CIL was found to be 6.4 min and that for METO is 4.3 min. The calibration was linear in concentration range of 1-10 µg/ml for CIL and that for METO was 5-25 µg/ml. The proposed method was validated in accordance with ICH parameters.
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