In the present study, the barks of Bridelia retusa Spreng, family: Euphorbiaceae were selected for phytochemical evaluation. Alcoholic extract was used for TLC and HPTLC study. Column chromatography was carried out for separation of phytoconstituent from alcoholic extract. In TLC study, alcoholic extract showed the presence of two coloured (pinkish) spots in toluene: methanol: formic acid (9: 1:0.5). After confirmation of the presence of sterol by TLC, the extract was subjected to isolation of sterol by column chromatography. The column chromatography was developed by using graded solvent mixtures of toluene and methanol. HPTLC study was carried out for alcoholic extract as well as for confirmation of isolated compound.
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