Two simple, precise and easy methods were successfully developed for the estimation of glimepiride in bulk and pharmaceutical dosage forms. The first method was based on UV-spectrophotometry, which is linear in the concentration range of 3-18 µg/ml at maximum absorbance (λ max) 228.4 nm. The correlation coefficient was found to be 0.9998. The accuracy of the method was ranged from 100.20 to 101.39%. The average percent relative standard deviation (%RSD) for precision was found to be 0.39%. The reproducibility of the method was determined based on ruggedness was found to be 0.84%. The second method was based on reversed-phase high-performance liquid chromatography (RP-HPLC) using methanol: phosphate buffer pH 4 (50:50 v/v) with 0.5 ml/min flow rate. The detector response was carried out at 229 nm. The average retention time for the drug was obtained 2.47 min. The calibration curves were linear from 10-50 µg/ml. The accuracy of the method was determined by percent recovery studies ranged from 98.6 to 99.9%. Mean intra - and inter-day assay relative standard deviations were 0.20 and 0.086%. Ruggedness and robustness were also calculated and found not more than 2%. The developed methods were applied successfully for the analysis of drug in its dosage forms and validated according to ICH guidelines.
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