The research into the separation of drug enantiomers is closely related to the safety and\nefficiency of the drugs. The aim of this study was to develop a simple and validated HPLC method\nto analyze cetirizine enantiomers. In the case of liquid dosage forms, besides the active substance\nin large amounts there are usually also inactive ingredients such as methyl- and propylparaben.\nUnfortunately, these compounds can interfere with the analyte, inter alia during chiral separation\nof the analyte enantiomers. The proposed innovative two-step liquid-liquid extraction procedure\nallowed for the determination of cetirizine enantiomers (along with M and P parabens) also in\nliquid dosage forms. The main focus of this study was the chromatographic activity of cetirizine\ndihydrochloride on the proteinate-based chiral stationary phase. The chromatographic separation\nof cetirizine enantiomers was performed on an immobilized human serum albumin (HSA) column\nfor the first time. Measurements were performed at a wavelength of 227 nm. Under optimal\nconditions, baseline separation of two enantiomers was obtained with 1.43 enantioseparation factor\n(�±) and 1.82 resolution (Rs). Finally, the proposed method was successfully applied to the selected\npharmaceutical formulations.
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