A simple, accurate and precise stability indicating RP-HPLC method was developed for the simultaneous estimation of dexamethasone sodium phosphate (DSP) and gatifloxacin (GA) in ophthalmic formulation. Method was achieved in various degradation condition like acidic, basic, oxidative, thermal and photolytic. Maximum degradation was found to be in acidic condition for DSP 29% and for GA in thermal condition. In developed method ODS-BP Hyperchrome C18 (250×4.6 mm, 5 µm) column and 0.02M Phosphate buffer:Methanol (60:40 % v/v) (pH-3.5 adjusted using 1% Orthophosphoric acid) mobile phase was used. The retention time for DSP and GA was found to be 3.37 min and 5.70 min. respectively. Accuracy data was found to be within the limit. The precision (intra‐day, inter‐day and repeatability) of method was found within limits. The methods were validated according to ICH guidelines for evaluation of accuracy, LOD and LOQ, precision, repeatability, reproducibility etc. The proposed method was successfully applied to determination of these drugs in ophthalmic formulation.
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