A new simple, sensitive and reproducible RP-HPLC method for the simultaneous estimation of hydrochlorothiazide and spiranolactone in pharmaceutical formulations was developed and validated. The separation was carried out on Phenomenex kinetex C18, 5μm (250×4.6 mm) column with acetonitrile: sodium dihydrogen and disodium hydrogen orthophasphate pH 6.4, 50:50% v/v as the mobile phase at the flow rate of 1.2 ml/min. The eluent detection was carried out using UV-Visible PDA detector at 254 nm. The retention time of hydrochlorothiazide and spiranolactone was 2.0 min and 5.7 min respectively. Linearity was observed hydrochlorothiazide and spiranolactone in the concentration range of 10-500 μg/ml and 10-5000 μg/ml respectively. The recovery of hydrochlorothiazide and spiranolactone was found to be 99.9.-100.2% and 100.2-100.8% respectively.
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