The stems of Dendrobium officinale Kimura et Migo (Dendrobii Officinalis Caulis) have a high medicinal value as a traditional\nChinesemedicine (TCM). Because of the limited supply, D. officinale is a high priced TCM, and therefore adulterants are commonly\nfound in the herbal market.Thedried stems of a closely related Dendrobium species, Dendrobium devonianum Paxt., are commonly\nused as the substitute; however, there is no effective method to distinguish the two Dendrobium species. Here, a high performance\nliquid chromatography (HPLC) method was successfully developed and applied to differentiate D. officinale and D. devonianum\nby comparing the chromatograms according to the characteristic peaks. A HPLC coupled with electrospray ionization multistage\nmass spectrometry (HPLC-ESI-MS) method was further applied for structural elucidation of 15 flavonoids, 5 phenolic acids, and 1\nlignan in D. officinale. Among these flavonoids, 4 flavonoid C-glycosides were firstly reported in D. officinale, and violanthin and\nisoviolanthin were identified to be specific for D. officinale compared with D. devonianum.Then, two representative components\nwere used as chemical markers. A rapid and reliable high performance thin layer chromatography (HPTLC) method was applied\nin distinguishing D. officinale from D. devonianum. The results of this work have demonstrated that these developed analytical\nmethods can be used to discriminate D. officinale and D. devonianum effectively and conveniently.
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