The present study was aimed to develop an analytical method of estimation of atenolol tablets using three different mixed co-solvents having varying composition of urea, PEG 400, hydroxy propyl β-cyclodextrin and PEG 6000. Beer’s law was obeyed in the concentration range of 4-24μg/ml. The solubility of atenolol in distilled water was found to be 9.34mg/ml, where as in mixed co-solvents it was 14.43 mg/ml in UPCD, 15.35 mg/ml in UPGP and 13.32 mg/ml in UPPE. The enhancement in solubility was observed to greater extent. The analysis of tablets indicated good correlation between estimated and label claim. The results of recovery study revealed that any small change in the drug concentration in the solution could be accurately determined by the proposed method. The low values of LOD and LOQ of acyclovir in the solvent mixture indicated good sensitivity of proposed method. As urea, PEG 400, PEF 6000 and HP-βCD were cheaper than most of the organic solvents, these can be used as a substitute for organic solvents. The study proved that mixed co-solvency phenomenon is an effective technique in enhancement of aqueous solubility of poorly water soluble drugs. The proposed method is new, simple, accurate, non-toxic and precise method that can be successfully employed for estimation of drugs in routine analysis of atenolol tablets.
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