A simple RP-HPLC method was developed and chemometric designs were applied for the simultaneous estimation of\nbacoside A3 (BA3), piperine (PPN) and crocin (CON) in polyherbal formulation brahmi vati. The separation was carried out by\nusing phenomenex C18 column (15 cm ââ?¬Â¢ 4.6 mm id, 5 Ã?¼m particle size). For the optimization the ranges of independent variables\nused were MeCN: 33ââ?¬â??38%, buffer conc.: 10ââ?¬â??20 mM and flow rate: 1ââ?¬â??2 ml/min. The data generated from the chromatograms\nwere mined by using chemometric methods such as trilinear regression analysis and cramerââ?¬â?¢s matrix method. The wavelengths\nselected for all the above methods were 248 nm (wavelength of maximum absorption; Ã?»max of BA3), 261 nm (wavelength of\nmaximum absorption; Ã?»max of PPN) and 274 nm (wavelength of maximum absorption; Ã?»max of CON). The method holds good\nlinearity for BA3 from 05-25 Ã?¼g/ml, for PPN from 10-50 Ã?¼g/ml and CON from 03-15 Ã?¼g/ml with regression coefficient values of\n0.999, 0.999 and 0.998 respectively. The intraday and inter-day precision was found to be less than 2% RSD. The percentage\nrecovery and percentage assay was in the range of 95-105% for bacoside A3 (BA3), piperine (PPN) and Crocin (CON) by all the\nmethods. The developed method neither requires any cumbersome separation procedure nor complex derivatization\nprocedures for the analysis of the three herbal drugs and moreover they are effective in minimizing the errors in analysis,\nsimple and economical.
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