Development and validation of UV Spectrophotometric method for simultaneous estimation of clotrimazole and beclomethasone dipropionate in their combined dosage form. First method is based on Q‐absorption Ratio method using two wavelengths, 260.6 nm (λmax of CT) and 250.8 nm (Isoabsorptive point). The second method is the dual wavelength method, where 237 nm and 241 nm were selected as λ1 and λ2 for the determination of Clotrimazole and 259 nm and 264 nm were selected similarly for the determination of Beclomethasone. The last method involves the use of First order derivative technique. Here 239 nm, the zero crossing point of Beclomethasone, was selected for the determination of Clotrimazole and 260.8 nm, the zero crossing point of Clotrimazole, was selected for the determination of Beclomethasone. Clotrimazole showed linearity in the range of 100‐450μg/mL and Beclomethasone showed linearity in the range of 6-34μg/mL in all the methods. All methods were validated statistically and recovery studies were carried out. All methods were found to be accurate, precise and reproducible. These methods were applied to the assay of the drugs in marketed formulation, which were found in therange of 98.0% to 100.0% of the labeled value for both Clotrimazole and Beclomethasone. Three new, simple, accurate and precise UV spectrophotometric methods have been developed and validated for the simultaneous determination of Clotrimazole (CT) and Beclomethasone dipropionate (BD) in their combined dosage forms.
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