A simple, rapid, accurate, precise and reproducible RP-HPLC method for the estimation of rizatriptan benzoate in bulk and tablet dosage form was developed and validated as per ICH guidelines. The separation was done using Enable C18 G 150 x 4.6 mm, 5µ column. The mobile phase (water and methanol; 80:20% v/v) was pumped at 1.0 ml/min and effluent was detected at 225 nm using a PDA detector. The retention time was 2.417 min and the method produced linear response in the concentration range of 1-10 µg/ml (r2 - 0.999). In recovery studies, %RSD from reproducibility was found to be below 2%. LOD and LOQ were 0.34 µg/ml and 1.036 µg/ml respectively. The developed RP-HPLC method was found to be effective, sensitive and specific for the estimation of rizatriptan benzoate in bulk and tablet dosage form.
Loading....