A reversed phase liquid chromatographic method with UV detection at 254 nm for dorzolamide assay in ophthalmic\nsolutions was developed and validated. Chromatographic separation was achieved on a Zorbax SB C18 (250mmÃ? 4.6 mm, ... column kept at ... with an isocratic mixture of mobile phase (phosphate buffer, pH 2.5, and acetonitrile, 90 : 10 v/v)\nat a flow rate of 0.8 mL/min. The method was validated for its specificity, linearity, accuracy, precision, limit of detection,\nlimit of quantification, and robustness based on ICH guidelines. The validation studies revealed satisfactory results. The\nproposed method has been applied for the quantification of dorzolamide in commercial samples. The developed method is\nfast, simple, specific, accurate, and sensitive, hence can be applied for routine quality control analysis of dorzolamide in\npharmaceutical dosage form.
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