In this study, the constituents of a Corydalis bungeana Turcz extract were qualitatively\nanalyzed using gradient elution with a mobile phase of 0.2% acetic acid and acetonitrile. We obtained\ncomprehensive insight into the constituents of C. bungeana Turcz extracts through the quantitative\nanalysis of 14 compounds by comparison with authentic reference standards, and tentatively identified\nan additional 44 compounds through electrospray ionization mass spectrometry (ESI-MS) and tandem\nMS detection. The separation was successfully achieved using an Agilent SB-C18 column (1.8 microm,\n150 * 2.1 mm; Agilent, Santa, CA, USA). A tandem quadrupole spectrometer was operated in either\nfull-scan mode or multiple reaction monitoring (MRM) for the qualitative and quantitative analyses\nof the constituents, respectively. Validation data (inter-day and intra-day combined) for accuracy and\nprecision ranged from -4.80% to 4.73%, and 0.30% to 4.97%, respectively. An ultrahigh performance\nliquid chromatographic-ESI-tandem MS (UHPLC-ESI-MS/MS) method for qualitative of C. bungeana\nTurcz (C. bungeana) extract and the quantification of 14 alkaloids, namely, A-N, was developed and\nvalidated. Quantitative analysis involved gradient elution with a mobile phase of 0.1% acetic acid\nand methanol for 45 min. The separation was successfully achieved using aWaters SB-C18 column\n(1.8 microm, 100 mm * 2.1 mm,Waters, Milford, Massachusetts, USA). The repeatability and stability of\nthe method also met USFDA criteria with CV values lower than 5%. The limit of detection of the\n14 alkaloids ranged from 9.74 to 13.00 ng/mL, whereas the linearities of the standard curves were\nbetween 0.9991 and 0.9995. In total, 15 commercial samples from 11 different sources were analyzed.
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